Lactose Colorimetric Assay Kit (E-BC-K753-M)
For research use only.
| Detection Principle |
Lactose is a disaccharide composed of galactose and glucose, serving as the primary source of carbohydrates during mammalian development. Lactose is catalyzed by galactosidase to produce galactose and glucose. The glucose is further converted by other enzymes, and the resulting product reacts with a chromogenic agent to form a colored substance with maximum absorption at 550 nm.The lactose content in the sample is determined by measuring the OD value at 550 nm and calculating from the standard curve. |
| Sample Type | Milk,Serum,Plasma,Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (540-560 nm) |
| Other Reagents Required | Distilled or deionized water, PBS (0.01 M, pH 7.4), 6 mol/L HCl, 6 mol/L NaOH |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 15.61 μmol/L |
| Detection Range | 15.61-2000 μmol/L |
| Precision | inter-assay CV: 3.2%-6.5% | intra-assay CV: 0.4%-1.0% |
| Sample Volume | 40 μL |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse kidney tissue homogenization | 1 |
| 10% Mouse liver tissue homogenization | 2-50 |
| 10% Mouse spleen tissue homogenization | 1 |
| 10% Mouse lung tissue homogenization | 1 |
| Bovine serum | 1 |
| Rat serum | 10-100 |
| Horse serum | 10-50 |
| Human serum | 1 |
| Human milk | 40-250 |
| 1×10^6 A549 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.sample_type}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
No files available. Please enter the correct identification code in the search area to query the relevant documents.
| Title | Status | Valid until | Action | |
|---|---|---|---|---|
| {{v.文件名}} | {{v.status_str}} | {{v.timestamp_str}} | Download | / |
*Note：Please download the relevant files within the validity period. After expiration, file preview and download will not be available on the page.
Contact with Us
Houston, Texas, 77079, USA
