Lactulose Fluorometric Assay Kit (E-BC-F049)

Name: Lactulose Fluorometric Assay Kit
Brand: Elabscience
SKU: E-BC-F049
Price: 520.0 USD
Availability: InStock

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Detection Principle	Lactulose (4-o-β-galactopyranosyl-D-fructo-furanose), also known as galactoside fructose, 4-β-D-galactosid-D-fructose and lactulose, contains one molecule of galactose and one molecule of fructose. Lactulose is a kind of synthetic disaccharide formed by base isomerization of lactose catalyzed by free amino group of casein during milk heat treatment. Lactulose can produce a specific product under the action of enzyme, which reacts with the chromogenic agent to produce a fluorescence product. The excitation wavelength is 530 nm, and the emission wavelength is 590 nm.
Sample Type	Dairy product,Feces
Detection Method	Fluorometric method
Detection Instrument	Fluorescence microplate reader (Ex/Em=530 nm/590 nm)
Research Area	Metabolic Diseases
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	4.78 μmol/L
Detection Range	4.78-500 μmol/L
Precision	inter-assay CV: 7.4-9.7% \| intra-assay CV: 2.6-3.8%
Sample Volume	50μL
Assay Time	2 h 30 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Fresh milk 1	5-10
Fresh milk 2	5-10
Pure milk	5-10
Infant feces	2-6

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.