Lactulose Fluorometric Assay Kit (E-BC-F049)
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For research use only.
| Detection Principle |
Lactulose (4-o-β-galactopyranosyl-D-fructo-furanose), also known as galactoside fructose, 4-β-D-galactosid-D-fructose and lactulose, contains one molecule of galactose and one molecule of fructose. Lactulose is a kind of synthetic disaccharide formed by base isomerization of lactose catalyzed by free amino group of casein during milk heat treatment. Lactulose can produce a specific product under the action of enzyme, which reacts with the chromogenic agent to produce a fluorescence product. The excitation wavelength is 530 nm, and the emission wavelength is 590 nm. |
| Sample Type | Dairy product,Feces |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=530 nm/590 nm) |
| Research Area | Metabolic Diseases |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 4.78 μmol/L |
| Detection Range | 4.78-500 μmol/L |
| Precision | inter-assay CV: 7.4-9.7% | intra-assay CV: 2.6-3.8% |
| Sample Volume | 50μL |
| Assay Time | 2 h 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Fresh milk 1 | 5-10 |
| Fresh milk 2 | 5-10 |
| Pure milk | 5-10 |
| Infant feces | 2-6 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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