Leucine Aminopeptidase (LAP) Activity Assay Kit (E-BC-K568-M)
For research use only.
| Detection Principle | LAP can catalyze the substrate L-leucine-4-nitroaniline to produce p-nitroaniline, which has the maximum absorption peak at the wavelength of 405 nm. The enzyme activity of LAP can be calculated by measuring the increasing OD value of the system. |
| Synonyms | LAP |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (405 nm) |
| Research Area | Liver And Renal Function |
| Other Reagents Required | Acetone |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 5.2 U/L |
| Detection Range | 5.2-201.8 U/L |
| Precision | inter-assay CV: 6.4% | intra-assay CV: 4.1% |
| Sample Volume | 10 μL |
| Assay Time | 25 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Dog serum | 1 |
| Rat serum | 1 |
| Cynomolgus monkey serum | 1 |
| 10% Rat spleen tissue homogenate | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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