LGALS4 Polyclonal Antibody (E-AB-19890)
For research use only.
| Verified Samples |
Verified Samples in WB: Human sigmoid, HT29 Verified Samples in IHC: Human esophagus cancer, Human thyroid cancer |
| Dilution | WB 1:500-1:2000, IHC 1:30-1:150 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide of human LGALS4 |
| Abbre | LGALS4 |
| Synonyms | Antigen NY CO 27, Antigen NY-CO-27, Antigen NYCO27, GAL 4, GAL4, Gal-4, Galectin 4, Galectin-4, Galectin4, Homo sapiens galectin4 mRNA complete cds, L 36 lactose binding protein, L-36 lactose-binding protein, L36 lactose binding protein, L36LBP, Lactose binding lectin |
| Swissprot | |
| Calculated MW | 36 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytosol, Extracellular region or secreted, extracellular space, Plasma Membrane. |
| Concentration | 0.96 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Epigenetics and Nuclear Signaling, Metabolism, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The expression of this gene is restricted to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer.LGALS4 (Galectin 4) is a Protein Coding gene. Diseases associated with LGALS4 include Paraneoplastic Cerebellar Degeneration and Colorectal Cancer. Among its related pathways are Cell adhesion_Cell-matrix glycoconjugates. GO annotations related to this gene include carbohydrate binding. An important paralog of this gene is LGALS9. |
Other Clones
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Unconjugated
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