Malic Acid Colorimetric Assay Kit (E-BC-K905-M)

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Detection Principle	L-malic acid, also known as 2-hydroxysuccinic acid, is widely present in living organisms. It has two stereoisomers and is one of the members of the tricarboxylic acid cycle, an important metabolic cycle in living organisms. The detection principle of this kit is that the enzyme catalyzes malic acid to produce oxaloacetic acid. At the same time, under the action of electronic coupler, the chromogenic agent is reduced to produce orange yellow product, which has the maximum absorption peak at about 450 nm.
Sample Type	Serum,Plasma,Tissue,Cell
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (445-465 nm, optimum wavelength: 450 nm)
Research Area	Tricarboxylic Acid（TCA）Cycle , Energy Metabolism
Other Reagents Required	Normal saline (0.9% NaCl)
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.003 mmol/L
Detection Range	0.003-0.5 mmol/L
Precision	inter-assay CV: 4.6-5.9% \| intra-assay CV: 2.6-3.6%
Sample Volume	40 μL

Cat.No.	Product Name	Sizes
E-BC-K804-M	NAD+/NADH Colorimetric Assay Kit (WST-8)	96T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Rat liver tissue homogenate	3-5
10% Mouse liver tissue homogenate	3-5
10% Lemon tissue homogenate	8-12
10% Tangerine tissue homogenate	5-10
10% Potato tissue homogenate	5-12
Rat serum	1
Mouse plasma	1
3.35 × 10^6 CHO cells	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.