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For research use only.

Verified Samples Verified Samples in WB: HUVEC
Dilution WB 1:500-1:2000,  IHC 1:100-1:300
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse,  Rat
Applications WB,  IHC-p
Clonality Polyclonal
Immunogen Synthesized peptide derived from the Internal region of human Mfn2.
Abbre Mfn2
Synonyms CMT2A,  CMT2A2,  CPRP 1,  CPRP1,  EC 3.6.5.-,  Fzo,  HSG,  Hypertension related protein 1,  KIAA0214,  MARF,  MFN 2,  MFN2,  Mfn2,  Mitochondrial assembly regulatory factor,  Mitofusin-2,  Mitofusin2,  Transmembrane GTPase MFN2,  hyperplasia suppressor gene
Swissprot
Calculated MW 86 kDa
Observed MW 86 kDa

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Mitochondrion outer membrane. Colocalizes with BAX during apoptosis.
Concentration 1 mg/mL
Buffer Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol.
Purification Method Affinity purification
Research Areas Cancer,  Cell Biology,  Metabolism,  Neuroscience,  Signal Transduction,  Tags and Cell Markers
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background This gene encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified.
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Unconjugated

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