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MIER1 Polyclonal Antibody (E-AB-92067)

All Size Price Qty
200μL $ 530.00
120μL $ 320.00
60μL $ 200.00
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For research use only.

Verified Samples Verified Samples in WB: various cell lines
Verified Samples in IHC: Mouse kidney
Dilution WB 1:500-1:2000,  IHC 1:50-1:200
Clonality Polyclonal
Immunogen Recombinant fusion protein of human MIER1
Synonyms ER1,  MI-ER1,  MIER1
Swissprot
Calculated MW 9 kDa/42-63 kDa
Observed MW 58 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm, nucleoplasm, nucleus.
Concentration 1 mg/mL
Buffer PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Purification Method Affinity purification
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background This gene encodes a protein that was first identified in Xenopus laevis by its role in a mesoderm induction early response (MIER). The encoded protein functions as a transcriptional regulator. Alternatively spliced transcript variants encode multiple isoforms, some of which lack a C-terminal nuclear localization signal.
Other Clones

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Unconjugated

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