Mini Sample Mouse CEACAM1 (Carcinoembryonic Antigen Related Cell Adhesion Molecule 1) ELISA Kit (E-MSEL-M0124)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CEACAM1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CEACAM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CEACAM1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 49 nm. The OD value is proportional to the concentration of CEACAM1 . You can calculate the concentration of CEACAM1 in the samples by comparing the OD of the samples to the standard curve.

Cell adhesion protein that mediates homophilic cell adhesion in a calcium-independent manner.Plays a role as coinhibitory receptor in immune response, insulin action and also functions as an activator during angiogenesis.Its coinhibitory receptor function is phosphorylation- and PTPN6 -dependent, which in turn, suppress signal transduction of associated receptors by dephosphorylation of their downstream effectors.Plays a role in immune response, of T-cells, natural killer (NK) and neutrophils.Upon TCR/CD3 complex stimulation, inhibits TCR-mediated cytotoxicity by blocking granule exocytosis by mediating homophilic binding to adjacent cells, allowing interaction with and phosphorylation by LCK and interaction with the TCR/CD3 complex which recruits PTPN6 resulting in dephosphorylation of CD247 and ZAP70.Also inhibits T-cell proliferation and cytokine production through inhibition of JNK cascade and plays a crucial role in regulating autoimmunity and anti-tumor immunity by inhibiting T-cell through its interaction with HAVCR2.