Mitochondrial Complex IV (Cytochrome C Oxidase ) Activity Assay Kit (E-BC-K152-M)
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For research use only.
| Detection Principle | Mitochondrial complex IV can catalyze the oxidation of reduced cytochrome C to oxidized cytochrome C, which has an absorption wavelength at 550 nm. Therefore, the activity of mitochondrial complex IV can be quantified by measure the change OD value at 550 nm. |
| Sample Type | Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (540-560 nm, optimum wavelength: 550 nm) |
| Research Area | Mitochondria Function , Cuproptosis , Energy Metabolism |
| Other Reagents Required | Anhydrous ethanol, PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 3.0 U/L |
| Detection Range | 3.0-88.6 U/L |
| Precision | inter-assay CV: 6.3-9.5 % | intra-assay CV: 3.2-5.0 % |
| Sample Volume | 30 μL(tissue homogenate) |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat brain tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1 |
| 10% Rat spleen tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
| 10% Mouse lung tissue homogenate | 1 |
| 10% Mouse spleen tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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