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Monkey FE (Ferritin) ELISA Kit (E-EL-MK2289)

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Citations ({{ citationsPage.numTotal }}) Manual MSDS
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All Size Price Qty
96T $ 682.00
48T $ 546.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary
Sensitivity 0.19 ng/mL
Detection Range 0.31-20 ng/mL
Sample Volume 100 μL
Total Assay Time 3 h 30 min
Reacitivity Monkey
Specificity This kit recognizes FE in samples.No significant cross-reactivity or interference between FE and analogues was observed.
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FE . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FE , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of FE . You can calculate the concentration of FE in the samples by comparing the OD of the samples to the standard curve.
FTH (Ferritin Heavy Chain 1, also Proliferation-Inducing Protein 15, PIG15, and PLIF) is a 183 aa cytosolic and secreted protein.Human FTH is highly conserved among species and shares 93% and 95% aa identity with mouse and rat FTH, respectively. Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
Uniport ID Q4R741
Research Area Cancer
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