Monoacylglycerol Lipase (MAGL) Activity Colorimetric Assay Kit (E-BC-K793-M)
For research use only.
| Detection Principle | Monoacylglycerol lipase (MAGL) is a widely distributed serine hydrolase. MAGL can not only decompose triacylglycerol, which plays an important role in lipid metabolism, but also hydrolyze 2-arachidonic acid and regulate the signaling of the cannabinoid system in the body. The principle of this kit is that the substrate is catalyzed by MAGL, and the product generated can react with the chromogenic agent. By measuring the amount of chromogenic substance produced per unit time, the MAGL enzyme activity in the sample can be calculated. |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (390-410 nm, optimum wavelength: 400 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Energy Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.05 U/L |
| Detection Range | 1.05-39.7 U/L |
| Precision | inter-assay CV: 3.3-10.2% | intra-assay CV: 1.8-5.0% |
| Sample Volume | 40 μL |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse kidney tissue homogenate | 50-500 |
| 10% Mouse heart tissue homogenate | 3-10 |
| 10% Mouse liver tissue homogenate | 50-500 |
| 10% Mouse lung tissue homogenate | 20-200 |
| 1×10^6 K562 cells | 1 |
| 1×10^6 Molt cells | 1 |
| Mouse plasma | 3-50 |
| Fetal bovine serum | 5-50 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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