Product Summary

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	100 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reacitivity	Mouse
Specificity	This kit recognizes ANG in samples.No significant cross-reactivity or interference between ANG and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ANG . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ANG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ANG , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 5 nm. The OD value is proportional to the concentration of ANG . You can calculate the concentration of ANG in the samples by comparing the OD of the samples to the standard curve.

Background

Mouse Angiogenin is a member of the pancreatic ribonuclease A superfamily and is a potent inducer of neovascularization. Furthermore, Angiogenin is essential for cell growth and proliferation. Angiogenin direct binding to the promoter region of ribosomal DNA induces ribosomal RNA transcription and cell proliferation required for ribosomal biogenesis and the action of angiogenic factors. Increased Angiogenin serum levels have been associated with the incidence and severity of several types of tumors. Mouse Angiogenin is 145 amino acids (aa) in length, with a 24 aa signal peptide, and contains three intra-chain disulfide bonds. Human and rat Angiogenin are 74% and 83% identical to mouse Angiogenin, respectively.

Gene Alias	ANG
Gene ID	11727
Uniport ID	P21570
Protein Alias	ANG
Research Area	Cancer

Mouse ANG (Angiogenin) ELISA Kit - procedures

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Size	Price	Qty
96T	$ 609.00	- +
48T	$ 487.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Mouse ANG (Angiogenin) ELISA Kit (E-EL-M3122)

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