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Mouse LDL(Low Density Lipoprotein) ELISA Kit (E-EL-M1363)

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Citations ({{ citationsPage.numTotal }}) Manual MSDS
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All Size Price Qty
96T $ 495.00
48T $ 396.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary
Sensitivity 4.69 ng/mL
Detection Range 7.81-500 ng/mL
Sample Volume 100 μL
Total Assay Time 3 h 30 min
Reacitivity Mouse
Specificity This kit recognizes Mouse LDL in samples. No significant cross-reactivity or interference between Mouse LDL and analogues was observed
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LDL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LDL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse LDL. You can calculate the concentration of Mouse LDL in the samples by comparing the OD of the samples to the standard curve.
Low-density lipoprotein is a kind of lipoprotein particles that carry cholesterol into peripheral tissue cells, which can be oxidized into oxidized low-density lipoprotein. When low-density lipoprotein, especially oxidized low-density lipoprotein (OX-LDL), is excessive, the cholesterol it carries will accumulate in the artery wall, which is easy to cause arteriosclerosis for a long time. That's why LDL is called the "bad cholesterol."
Research Area Cancer , Cardiovascular , Metabolism , Signal Transduction
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