Mouse TNNI3/cTn-I (Troponin I Type 3, Cardiac) CLIA Kit (E-CL-M0668)

Name: Mouse TNNI3/cTn-I (Troponin I Type 3, Cardiac) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0668
Price: 180.0 USD
Availability: InStock

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Mouse TNNI3/cTn-I (Troponin I Type 3, Cardiac) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	1.88 pg/mL
Detection Range	3.13-200 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse TNNI3/cTn-I in samples.No significant cross-reactivity or interference between Mouse TNNI3/cTn-I and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
200	41525.000	48017.000	44771.000	44733
100	17668.000	19290.000	18479.000	18441
50	9621.000	8195.000	8908.000	8870
25	3810.000	4066.000	3938.000	3900
12.5	2215.000	2137.000	2176.000	2138
6.25	1215.000	1037.000	1126.000	1088
3.13	598.000	608.000	603.000	565
0	38.000	38.000	38.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	10.730	18.040	72.270	11.340	18.220	78.210
Standard deviation	0.860	1.720	6.640	1.220	2.070	7.360
CV（%）	8.010	9.530	9.190	10.760	11.360	9.410

Recovery

The recovery of Mouse TNNI3 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	93-109	101
EDTA plasma （n=8）	92-108	100
Cell culture media （n=8）	94-109	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse TNNI3 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	90-104	89-101	97-115
1:2	Average	95	96	105
1:4	Range	94-108	103-119	86-99
1:4	Average	101	109	91
1:8	Range	86-100	88-104	97-110
1:8	Average	93	95	103

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNNI3/cTn-I. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TNNI3/cTn-I and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNNI3/cTn-I, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse TNNI3/cTn-I. You can calculate the concentration of Mouse TNNI3/cTn-I in the samples by comparing the RLU value of the samples to the standard curve.

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