Myeloperoxidase (MPO) Peroxidation Activity Fluorometric Assay Kit (E-BC-F013)
For research use only.
| Detection Principle | Under the catalysis of peroxidase, hydrogen peroxide can oxidize the non-fluorescent probe into the fluorescent substance, and its fluorescence intensity is proportional to the total peroxidase activity in the sample. This kit specifically inhibits the peroxidase activity of MPO in the sample through an MPO enzyme inhibitor, thus distinguishing the peroxidase activity of MPO in the sample from that of other peroxidases. |
| Synonyms | MPO |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Oxidative Stress |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.001 U/L |
| Detection Range | 0.001-1.26 U/L |
| Precision | inter-assay CV: 5.4% | intra-assay CV: 0.01 |
| Assay Time | 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Porcine serum | 5-10 |
| Rabbit serum | 3-5 |
| Rat serum | 2-5 |
| Mouse serum | 10-20 |
| Mouse plasma | 30-50 |
| Horse serum | 2-5 |
| 10% Rat heart tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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