N-Acetylcysteine (NAC) Fluorometric Assay Kit (E-BC-F025)
For research use only.
| Detection Principle |
N-Acetylcysteine (NAC) is a precursor for the biosynthesis of cysteine and glutathione. It is an antioxidant and free radical scavenger, commonly used in the treatment of various diseases. The principle of this kit is as follows: the NAC probe reacts with NAC to generate fluorescence, which can be measured at excitation and emission wavelengths of 510 nm and 560 nm, respectively. The NAC content in samples is then quantified by comparing the fluorescence values with those of NAC standards. |
| Sample Type | Serum,Plasma,Tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=510 nm/560 nm) |
| Research Area | Oxidative Stress |
| Other Reagents Required | Double distilled water |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.057 μmol/L |
| Detection Range | 0.057-1 μmol/L |
| Precision | inter-assay CV: 5.9%-8.2% | intra-assay CV: 1.0%-2.5% |
| Sample Volume | 20 μL |
| Assay Time | 50min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 1×10^6 HepG2 cells | 20–30 |
| 1×10⁶ RAW264.7 cells | 20–30 |
| Human serum | 2–10 |
| Mouse serum | 2–5 |
| Rat serum | 2–5 |
| Rabbit serum | 2–5 |
| 10% mouse liver homogenate | 80–100 |
| 10% mouse kidney homogenate | 80–100 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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