NANOG Polyclonal Antibody (E-AB-70199)
For research use only.
| Verified Samples |
Verified Samples in WB: Mouse brain, Mouse ovary, Rat brain Verified Samples in IHC: Mouse testis, Rat testis |
| Dilution | WB 1:500-1:2000, IHC 1:300-1:800 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse Nanog |
| Abbre | NANOG |
| Synonyms | ENK, Embryonic stem cell specific homeobox protein (Nanog), FLJ12581, Homeobox protein NANOG, Homeobox transcription factor Nanog, NANOG, Nanog homeobox, hNanog, homeobox transcription factor Nanog-delta 48 |
| Swissprot | |
| Calculated MW | 42 kDa |
| Observed MW |
42 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus. |
| Concentration | 430 μg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 1% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling, Developmental Biology, Stem Cells |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency.The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells.Two transcript variants encoding different isoforms have been found for this gene. |
Other Clones
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Other Formats
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Unconjugated
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