Nitrite Colorimetric Assay Kit (E-BC-K070-S)
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For research use only.
| Detection Principle | Nitrite can react with chromogenic agent producing light red azo-compound. The content of nitrite can be calculated indirectly by measuring the OD value at 550 nm. |
| Sample Type | Serum,Plasma,Saliva,Tissue,Cell,Cell culture supernatant |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (550 nm) |
| Research Area | Oxidative Stress |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Sensitivity | 1.36 μmol/L |
| Detection Range | 1.36-500 μmol/L |
| Precision | inter-assay CV: 3.3% | intra-assay CV: 2.6% |
| Sample Volume | 0.2-0.4 mL |
| Assay Time | 1 h |
| Cat.No. | Product Name | Clone No. |
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