One-component TMB Substrate (E-IR-R201)
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For research use only.
| Applications |
ELISA
|
| Storage |
2-8℃
|
| Expiration date |
12 months
|
| Introduction |
TMB is a popular chromogenic substrate for HRP detection in ELISA and is available in several formats. The soluble One-component TMB Substrate are supplied as Ready-to-Use mono-component liquids that require no preparation before use. The product mainly contains 3,3’,5,5’-tetramethylbenzidine and contains no DMF or DMSO.
One-component TMB Substrate yields a blue color Amax= 370 nm and 652 nm) when reacted with peroxidase in microwell applications. The product changes to yellow Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.
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