PARD3 Polyclonal Antibody (E-AB-40193)
For research use only.
| Verified Samples |
Verified Samples in WB: Rat brain |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Rat |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant Rat Partitioning defective 3 homolog protein |
| Abbre | PARD3A |
| Synonyms | ASIP, Atypical PKC isotype specific interacting protein, Atypical PKC isotype-specific-interacting protein, Baz, CTCL tumor antigen se2-5, Coagulation factor II receptor-like 2, F2rl2, PAR-3, agouti signaling protein, bazooka, par-3 family cell polarity regulator a |
| Swissprot | |
| Calculated MW | 151 kDa |
| Observed MW |
151 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Endomembrane system. Cell junction. Cell junction>tight junction. Cell membrane. Cytoplasm>cell cortex. Cytoplasm>cytoskeleton. Localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. Colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II (By similarity). Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction. |
| Concentration | 2 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Neuroscience, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This gene encodes a member of the PARD protein family. PARD family members interact with other PARD family members and other proteins; they affect asymmetrical cell division and direct polarized cell growth. Multiple alternatively spliced transcript variants have been described for this gene.PARD3 (Par-3 Family Cell Polarity Regulator) is a Protein Coding gene. Diseases associated with PARD3 include Gamma Heavy Chain Disease and Enamel Caries. Among its related pathways are Transcriptional activity of SMAD2/SMAD3-SMAD4 heterotrimer and Ras signaling pathway. GO annotations related to this gene include protein phosphatase binding and phosphatidylinositol-4,5-bisphosphate binding. An important paralog of this gene is PARD3B. |
Other Clones
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Other Formats
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Unconjugated
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