PCNA Monoclonal Antibody (AN005350L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HT-29, Jurkat, THP-1, Mouse spleen, Raw264.7, C6, HeLa, NIH/3T3 Verified Samples in IP: THP-1 Verified Samples in IHC: Human lung cancer, Human ovary cancer |
| Dilution | WB 1:2000-1:4000, IP 4ug/sample, IHC 1:200-1:400 |
| Isotype | IgG2a |
| Host | Mouse |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IP, IHC |
| Clonality | Monoclonal |
| Immunogen | Recombinant human PCNA protein expressed by E.coli |
| Abbre | PCNA |
| Synonyms | Proliferating cell nuclear antigen,PCNA,Cyclin |
| Swissprot | |
| Calculated MW | 29 kDa |
| Observed MW |
33 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Research Areas | Cell Biology, Epigenetics and Nuclear Signaling, Tags & Cell Markers, Cancer, Isotype, Loading Controls |
| Clone No. | 9C6 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
| background | Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways.Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. |
Other Clones
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