PD-L1/B7-H1/CD274 Monoclonal Antibody (AN200150P)
For research use only.
| Verified Samples |
Verified Samples in WB: K562, THP1, Raji Verified Samples in IP: K562 |
| Dilution | WB 1:500-1:2000, IP 1-4 μL/mg of lysate |
| Isotype | IgG1 |
| Host | Mouse |
| Reactivity | Human |
| Applications | WB |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human PD-L1/B7-H1/CD274 Protein |
| Abbre | CD274 |
| Synonyms | PDL, B7H, PD-L, PD1L, Programmed cell death 1 ligand, PDCD1L, PDCD1LG, B7-H, B7H1, PDL1, PD-L1, PDCD1L1, PDCD1LG1, CD274, B7-H1, B7 homolog 1, CD274 molecule, PDCD1 ligand 1, Programmed death ligand 1, PD1L1, Programmed cell death 1 ligand 1, PDL1, PD-L1, B7-H, B7H1, CD274 molecule, PDCD1L1, PDCD1LG1, B7 H, B7 H1, CD 274, CD274 antigen, MGC142294, MGC142296, OTTHUMP00000021029, PD L1, PDL 1, RGD1566211 |
| Swissprot | |
| Calculated MW | 33 kDa |
| Observed MW |
45 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Immunology, Cancer |
| Clone No. | 10D2 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | Human B7 homolog 1 (B7-H1), also called programmed death ligand 1 (PD-L1) and programmed cell death 1 ligand 1 (PDCD1L1), is a member of the growing B7 family of immune proteins that provide signals for both stimulating and inhibiting T cell activation. Other family members include B7-1, B7-2, B7-H2, PDL2 and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily. |
Other Clones
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Unconjugated
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