Pectinase Activity Colorimetric Assay Kit (E-BC-K867-M)
Add to cart
For research use only.
| Detection Principle |
Pectinase is an enzyme that breaks down pectin. Depending on the different substrates it acts upon, it can be classified into three types: pectin esterase, polygalacturonic acidase and pectin lyase. It is widely present in higher plants and microorganisms and is widely used in the food industry. The detection principle of this kit: The substances generated by pectinase hydrolyzing pectin react with the chromogenic agent upon heating to form a brown-colored substance. The activity of pectinase is calculated by measuring the change in absorbance at 540 nm. |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader 5(530-560 nm, optimum wavelength: 540 nm) |
| Research Area | Plant Physiology Series |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.15 U/mL |
| Detection Range | 0.15-20 U/mL |
| Precision | inter-assay CV: 3.6-7.2% | intra-assay CV: 1.1-1.4% |
| Sample Volume | 50 μL(tissue/cell homogenate) |
| Assay Time | 1 h 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Tomato pulp tissue homogenate | 5-10 |
| 10% Navel orange pulp tissue homogenate | 5-10 |
| 10% Banana peel tissue homogenate | 2-10 |
| 10% Apple pulp tissue homogenate | 10-20 |
| 10% Navel orange peel tissue homogenate | 10-20 |
| 10% Corn seed tissue homogenate | 2-5 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
| Cat.No. | Product Name | Clone No. |
|---|
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.sample_type}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
