Peroxidase (POD) Activity Fluorometric Assay Kit (E-BC-F012)
For research use only.
| Detection Principle |
Peroxidase (POD) is a class of oxidoreductases widely distributed in animals, plants, and microorganisms. It catalyzes the oxidation of various substrates by hydrogen peroxide (H2O2 ) or organic peroxides, while acting as an electron acceptor. POD catalyzes the substrate to produce a fluorescent product, and POD activity is calculated by measuring the change in fluorescence intensity of the product at an excitation wavelength of 535 nm and an emission wavelength of 587 nm per unit time. |
| Synonyms | HRP |
| Sample Type | Serum,Plasma,Animal tissue,Plant tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Other Reagents Required | PBS(0.01 M，pH = 7.4),double distilled water |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.0032 U/L |
| Detection Range | 0.0032-3 U/L |
| Precision | inter-assay CV: 7.6%-10.0% | intra-assay CV: 1.2%-2.0% |
| Sample Volume | 20 μL |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Porcine liver tissue homogenate | 6-10 |
| 10% Mouse liver tissue homogenate | 6-10 |
| 10% Mouse spleen tissue homogenate | 1 |
| 10% Chinese yam tissue homogenization | 3-8 |
| 10% Chili pepper flesh tissue homogenization | 8-15 |
| 10% Orange peel tissue homogenization | 1 |
| 10% Apple flesh tissue homogenization | 1 |
| Rat serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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