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PFDN1/Prefoldin subunit 1 Monoclonal Antibody (AN200147P)

All Size Price Qty
100μL $ 320.00
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For research use only.

Verified Samples Verified Samples in WB: 293T, Hela, Jurkat
Dilution WB 1:500-1:2000,  
Isotype IgG1
Host Mouse
Reactivity Human
Applications WB
Clonality Monoclonal
Immunogen Recombinant Human PFDN1/Prefoldin subunit 1 Protein
Abbre PFDN1
Synonyms PFD,  PFDN,  PFDN1,  PDF,  PFD1
Swissprot
Calculated MW 14 kDa
Observed MW 14 kDa

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Concentration 1 mg/mL
Buffer 0.2 μm filtered solution in PBS
Purification Method Protein A
Research Areas Signal Transduction,  Epigenetics and Nuclear Signaling
Clone No. 10C6
Conjugation Unconjugated
Storage This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Shipping Ice bag
background This gene encodes a member of the prefoldin beta subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils.
Other Clones

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Unconjugated

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