Phospho-AMPK alpha1/2 (Thr183/172) Polyclonal Antibody (E-AB-21121)
For research use only.
| Verified Samples |
Verified Samples in WB: L929, Rat kidney, Mouse brain Verified Samples in IHC: Mouse brain Verified Samples in IF: Rat heart |
| Dilution | WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat, Monkey |
| Applications | WB, IHC-p, IF |
| Clonality | Polyclonal |
| Immunogen | Synthesized peptide derived from human AMPKα1/2 around the phosphorylation site of Thr183/172 |
| Abbre | AMPKα1/2 (phospho Thr183/172) |
| Synonyms | 5'-AMP-activated protein kinase catalytic subunit alpha-1, ACACA kinase, AMPK, AMPK subunit alpha-1, AMPK1, Acetyl-CoA carboxylase kinase, HMGCR kinase, Hydroxymethylglutaryl-CoA reductase kinase, PRKAA1, PRKAA2, Tau-protein kinase PRKAA1 |
| Swissprot | |
| Calculated MW | 62 kDa |
| Observed MW |
63 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytosol, Nucleus, nuclear speck, nucleoplasm, nucleus, Plasma Membrane, apical plasma membrane, Other locations: cytoplasm, intracellular, nucleotide-activated protein kinase complex. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cardiovascular, Metabolism, Neuroscience, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation.Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. |
Other Clones
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Unconjugated
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