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Phospho-Catenin beta (Ser37) Polyclonal Antibody (E-AB-20828)

All Size Price Qty
200μL $ 399.00
120μL $ 240.00
60μL $ 143.00
20μL $ 73.00
Add to cart

For research use only.

Verified Samples Verified Samples in WB: 293T
Verified Samples in IF: Human breast cancer, Human kidney
Dilution WB 1:500-1:2000,  IHC 1:100-1:300,  IF 1:200-1:1000
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse,  Rat
Applications WB,  IHC-p,  IF
Clonality Polyclonal
Immunogen Synthesized peptide derived from human Catenin-β around the phosphorylation site of Ser37
Synonyms 88kDa,  Beta catenin,  Beta-catenin,  CATNB,  CHBCAT,  CTNB1,  CTNNB,  CTNNB1,  Cadherin associated protein,  Catenin (cadherin associated protein),  Catenin beta 1,  Catenin beta-1,  DKFZp686D02253,  FLJ25606,  FLJ37923,  OTTHUMP00000162082,  OTTHUMP0000016522,  OTTHUMP00000165222,  beta 1
Swissprot
Calculated MW 85 kDa
Observed MW 85 kDa

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm. Nucleus. Cytoplasm>cytoskeleton. Cell junction>adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
Tissue Specificity Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Purification Method Affinity purification
Research Areas Cancer,  Cardiovascular,  Neuroscience,  Signal Transduction,  Stem Cells
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
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Unconjugated

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