Phospho-FAK (Tyr397) Polyclonal Antibody (E-AB-21207)

For research use only.
Verified Samples |
Verified Samples in WB: 293T Verified Samples in IHC: Human stomach cancer, Mouse brain |
Dilution | WB 1:500-1:2000, IHC 1:100-1:300 |
Isotype | IgG |
Host | Rabbit |
Reactivity | Human, Mouse, Rat |
Applications | WB, IHC-p |
Clonality | Polyclonal |
Immunogen | Synthesized peptide derived from human FAK around the phosphorylation site of Tyr397 |
Synonyms | FADK, FADK 1, FAK related non kinase polypeptide, FAK1, FRNK, Focal adhesion Kinase, Focal adhesion kinase 1, Focal adhesion kinase isoform FAK Del33, Focal adhesion kinase related nonkinase, PPP1R71, Protein phosphatase 1 regulatory subunit 7, p125FAK, pp125FAK |
Swissprot | |
Calculated MW | 119 kDa |
Observed MW |
119 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Cell junction>focal adhesion. Cell membrane. Constituent of focal adhesions. |
Tissue Specificity | Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain. |
Concentration | 1 mg/mL |
Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
Purification Method | Affinity purification |
Research Areas | Cancer, Cardiovascular, Signal Transduction |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
background | Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity. |
Other Clones
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Unconjugated
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