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Phospho-MAP3K7 (Thr187) Polyclonal Antibody (E-AB-21065)

All Size Price Qty
200μL $ 399.00
120μL $ 240.00
60μL $ 143.00
20μL $ 73.00
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For research use only.

Verified Samples Verified Samples in WB: 293T
Dilution WB 1:500-1:2000,  IHC 1:100-1:300
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse,  Rat
Applications WB,  IHC-p
Clonality Polyclonal
Immunogen Synthesized peptide derived from human Tak1 around the phosphorylation site of Thr187
Synonyms M3K7,  MAP3K 7,  MEKK7,  Map3k7,  Mitogen activated protein kinase kinase kinase 7,  Mitogen-activated protein kinase kinase kinase 7,  TAK1,  TGF beta activated kinase 1,  TGF-beta-activated kinase 1,  TGF1a,  Transforming ,  Transforming growth factor beta activated kinase 1
Swissprot
Calculated MW 67 kDa
Observed MW 60 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytosol, Endosome membrane, Nucleus, Ada2/Gcn5/Ada3 transcription activator complex, nucleus, Plasma Membrane.
Tissue Specificity Isoform 1A is the most abundant in ovary, skeletal muscle, spleen and blood mononuclear cells. Isoform 1B is highly expressed in brain, kidney and small intestine. Isoform 1C is the major form in prostate. Isoform 1D is the less abundant form.
Concentration 1 mg/mL
Buffer Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol.
Purification Method Affinity purification
Research Areas Cancer,  Cardiovascular,  Immunology,  Signal Transduction
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background Component of a protein kinase signal transduction cascade. Mediator of TRAF6 and TGF-beta signal transduction. Activates IKBKB and MAPK8 in response to TRAF6 signaling. Stimulates NF-kappa-B activation and the p38 MAPK pathway. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK, but not that of NF-kappa-B.
Other Clones

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Other Formats

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Unconjugated

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