Phospho-SMAD2/3 (Thr8) Polyclonal Antibody (E-AB-21040)
For research use only.
| Verified Samples |
Verified Samples in WB: VEC |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Synthesized peptide derived from human Smad2/3 around the phosphorylation site of Thr8 |
| Abbre | Smad2/3 (phospho Thr8) |
| Synonyms | JV18-1, MAD homolog 2, MADH2, MADH3, MADR2, Mad-related protein 2, Mothers against DPP homolog 2, Mothers against decapentaplegic, Mothers against decapentaplegic homolog 2, SMAD 2, SMAD family member 2, SMAD2, SMAD3, Smad2, hMAD-2, hSMAD2 |
| Swissprot | |
| Calculated MW | 48 kDa |
| Observed MW |
48 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytosol, Nucleus, heteromeric SMAD protein complex, nuclear chromatin, nuclear inner membrane, nucleoplasm, nucleus, Plasma Membrane, Other locations: cytoplasm, receptor complex, SMAD protein complex, transcription factor complex. |
| Tissue Specificity | SMAD2 is Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling, Signal Transduction, Stem Cells |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. |
Other Clones
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Other Formats
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Unconjugated
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