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Phospho-STAT4 (Tyr693) Polyclonal Antibody (E-AB-20984)

All Size Price Qty
200μL $ 399.00
120μL $ 240.00
60μL $ 143.00
20μL $ 73.00
Add to cart

For research use only.

Verified Samples Verified Samples in WB: COS-7
Dilution WB 1:500-1:2000,  IHC 1:100-1:300,  IP 1:200-1:500
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse,  Rat,  Monkey
Applications WB,  IHC-p,  IP
Clonality Polyclonal
Immunogen Synthesized peptide derived from human Stat4 around the phosphorylation site of Tyr693
Synonyms SLEB11,  STAT4,  Signal transducer and activator of transcription 4
Swissprot
Calculated MW 86 kDa
Observed MW 86 kDa

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Purification Method Affinity purification
Research Areas Cancer,  Epigenetics and Nuclear Signaling,  Signal Transduction
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.
Other Clones

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Unconjugated

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