Phosphocreatine (PCr) Fluorometric Assay Kit (E-BC-F154)
Add to cart
For research use only.
| Detection Principle |
Phosphocreatine (PCr) is a high-energy phosphate compound that mainly exists in muscle and nerve tissues and is an important substance in the rapid energy supply system of living organisms. It plays a buffering and energy storage role in cellular energy metabolism through its interaction with adenosine triphosphate (ATP), especially providing immediate energy to muscles during short-term high-intensity exercise. The detection principle of this kit: The products generated by PCr through a series of enzymatic reactions cause the fluorescent probe to produce fluorescence. Fluorescence can be detected at an excitation wavelength of 535 nm and an emission wavelength of 587 nm. The concentration of PCr in the sample is calculated based on the magnitude of the fluorescence value. |
| Sample Type | Animal tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Energy Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.17 μmol/L |
| Detection Range | 1.17-100.00 μmol/L |
| Precision | inter-assay CV: 8.3-9.7% | intra-assay CV: 1.3-3.5% |
| Sample Volume | 20 μL(tissue homogenate/cell homogenate) |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse muscle tissue homogenate | 1-4 |
| 10% Mouse brain tissue homogenate | 1-3 |
| 1×10^6 RAW 264.7 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
| Cat.No. | Product Name | Clone No. |
|---|
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.sample_type}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
