Phosphoglycerate Kinase (PGK) Activity Colorimetric Assay Kit (E-BC-K778-M)

Name: Phosphoglycerate Kinase (PGK) Activity Colorimetric Assay Kit
Brand: Elabscience
SKU: E-BC-K778-M
Price: 310.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Resources

Phosphoglycerate Kinase (PGK) Activity Colorimetric Assay Kit - 1

All	Size	Price	Qty
	96T	$ 460.00	- +
	48T	$ 310.00	- +

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Detection Principle	Phosphoglycerate kinase (PGK) is an enzyme that plays a key role in glycolysis and operates in the second stage of the glycolytic pathway. Catalyze the reversible transfer of phosphate groups from 1, 3-diphosphoglycerate (1, 3-DPG) to ADP, thereby generating 3-phosphoglycerate (3-PGA) and ATP. PGK is an indispensable key enzyme in living organisms, and its protein kinase activity plays an important role in maintaining cellular homeostasis. The determination principle of this kit: PGK catalyzes the substrate reaction and consumes the reducing agent. The reducing agent has the maximum absorbance at 340 nm. The enzyme activity of PGK is calculated by calculating the amount of reducing agent consumed per unit time of the reaction.
Sample Type	Animal tissue,Cell,Serum,Plasma
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (340 nm)
Research Area	Glycolysis And Lipid Metabolism
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	3.22 U/L
Detection Range	3.22-391.32 U/L
Precision	inter-assay CV: 2.5%-6.5% \| intra-assay CV: 1.6%-2.2%
Sample Volume	20 μL
Assay Time	30 min

Cat.No.	Product Name	Sizes
E-BC-F078	Mitochondrial Stress Fluorometric Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Rat plasma	1
Mouse serum	1
Human serum	1
10% Mouse liver tissue homogenate	20-40
10% Mouse kidney tissue homogenate	20-40
10% Mouse muscle tissue homogenate	20-40
1×10^6 HL-60 cells	1-3
1×10^6 293T cells	1-5
1×10^6 Jurkat cells	1-3

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.