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Phytic Acid Assay Kit - 1
  • Phytic Acid Assay Kit - 1
  • Phytic Acid Assay Kit - 2
  • Phytic Acid Assay Kit - 3
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96T $ 380.00
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For research use only.

Detection Principle Phytic acid, also known as inositol hexaphosphate, is widely found in cereals, beans, and vegetables. The detection principle of this kit: Phytic acid generates inorganic phosphorus under the catalysis of enzyme. Under acidic conditions, inorganic phosphorus reacts with ammonium molybdate to produce blue molybdenum blue substance, which has a characteristic absorption peak at 700 nm. By measuring the content of inorganic phosphorus, the phytic acid content can be calculated.
Sample Type Plant tissue,Liquid
Detection Method Colorimetric method
Detection Instrument Microplate reader (690-705 nm, optimum wavelength: 700 nm)
Research Area Plant Physiology Series
Storage This product can be stored at 2-8°C/-20℃ for 12 months with shading light.
Valid Period 12 months
Sensitivity 3.78 nmol/mL
Detection Range 3.78-500 nmol/mL
Precision inter-assay CV: 4.5-6.0% | intra-assay CV: 2.3-4.1%
Sample Volume 240 μL
Assay Time 3 h

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
10% Tomato tissue homogenate 1
10% Carota tissue homogenate 1
10% Green pepper tissue homogenate 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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Biotin

Chromogenic Biotin

Cyanine 3

Cyanine 5

Cyanine 5.5

Cyanine 7

Elab Fluor® 430

Elab Fluor® 488

Elab Fluor® 568

Elab Fluor® 594

Elab Fluor® 647

Elab Fluor® 680

Elab Fluor® 700

Elab Fluor® 750

Elab Fluor® Red 780

Elab Fluor® Violet 450

Elab Fluor®488

Elab Fluor®594

Elab Fluor®647

Elab Fluor®Violet 450

FITC

HRP

NHS-Biotin

NHS-LC-LC-Biotin

Sulfo-NHS-Biotin

Sulfo-NHS-LC-LC-Biotin

Unconjugated

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