Phytase Assay Kit (E-BC-K870-M)

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	96T	$ 360.00	- +

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Detection Principle	Phytase also known as inositol hexaphosphatase, is a protein and sugar binding enzyme. Phytase can catabolize phytic acid to produce inorganic phosphorus and inositol, which greatly improves nutrient utilization rate of organisms. Natural phytase is widely found in plants, animal tissues and microorganisms. Phytase has a wide research value in the field of food production and animal husbandry. Under certain environmental conditions, phytase can decompose sodium phytate to produce inorganic phosphorus and inositol derivatives. Under acidic conditions, inorganic phosphorus and ammonium molybdate chromogenic agent react to produce a blue molybdenum blue substance, which has a characteristic absorption peak at 700 nm. Phytase activity can be calculated by measuring the content of inorganic phosphorus.
Sample Type	Animal and Plant tissue,Microorganism
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (690-710 nm, optimum wavelength: 700 nm)
Research Area	Plant Physiology Series
Other Reagents Required	Concentrated sulfuric acid (98%)
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	1 U/mL
Detection Range	1 - 170 U/mL
Precision	inter-assay CV: 3.6-5.0% \| intra-assay CV: 3.2-4.5%
Sample Volume	100 μL
Assay Time	50 min

Cat.No.	Product Name	Sizes
E-BC-K245-M	Phosphorus (Pi) Colorimetric Assay Kit (Phospho Molybdate Method)	96T , 48T
E-BC-K245-S	Phosphorus (Pi) Colorimetric Assay Kit (Phospho Molybdate Method)	100Assays , 50Assays
E-BC-K869-M	Phytic Acid Assay Kit	96T