Phytic Acid Colorimetric Assay Kit (E-BC-K1210-M)
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For research use only.
| Detection Principle |
Phytic Acid is the main source of inositol and stored phosphorus in plant seeds, accounting for up to 90% of the total phosphorus. The detection principle of this kit: Enzymes can decompose phytic acid to produce inorganic phosphorus and inositol derivatives. The generated inorganic phosphorus reacts with the molybdate ammonium chromogenic agent, and the increase in OD value at 660 nm can be used to calculate the content of phytic acid. |
| Sample Type | Liqid,Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (650-670 nm, optimum wavelength: 660 nm) |
| Research Area | Plant Physiology Series |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 6.05 μmol/L |
| Detection Range | 6.05-500 μmol/L |
| Precision | inter-assay CV: 3.5%-4.8% | intra-assay CV: 1.2%-2.4% |
| Sample Volume | 10 μL(tissue homogenate) |
| Assay Time | 1 h 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Cinnamon leave tissue homogenate | 1 |
| 10% Scindapsus aureus leaf tissue homogenate | 1 |
| 10% Horse chestnut leaf tissue homogenate | 1 |
| 10% Heath leave tissue homogenate | 1 |
| 10% Ligustrum leaf tissue homogenate | 1 |
| 10% Small wax leave tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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