For research use only.
| Verified Samples |
Verified Samples in WB: 293T Verified Samples in IHC: Human prostate cancer |
| Dilution | WB 1:500-1:2000, IHC 1:10-1:50 |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide of human PIBF1 |
| Abbre | PIBF1 |
| Synonyms | C13orf24, KIAA1008, PIBF, PIBF 1, PIBF1, Progesterone immunomodulatory binding factor 1, Progesterone induced blocking factor 1, Progesterone-induced-blocking factor 1, RP11 505F3.1 |
| Swissprot | |
| Calculated MW | 90 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoskeleton, centriolar satellite, centrosome, microtubule organizing center, Cytosol, Extracellular region or secreted, extracellular space, Nucleus, nucleoplasm, nucleus. |
| Concentration | 0.5 mg/mL |
| Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Purification Method | Antigen affinity purification |
| Research Areas | Cell Biology, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Progesterone-induced-blocking factor 1 is a protein that in humans is encoded by the PIBF1 gene. It has been shown to localize to the centrosome and has also been named CEP90. Mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling NK activity exerts an anti-abortive effect. |
Other Clones
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