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Plant Ammonium Nitrogen Colorimetric Assay Kit (E-BC-K859-M)

All Size Price Qty
96T $ 240.00
48T $ 150.00
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For research use only.

Detection Principle Ammonium nitrogen reacts with hypochlorite and phenolic substances in a strong alkaline medium to produce water-soluble dye indigophenol blue, which has a characteristic absorption peak at 625nm, and the absorption value is proportional to the content of ammonium nitrogen.
Sample Type Plant tissue
Detection Method Colorimetric method
Detection Instrument Microplate reader (615-635 nm, optimum wavelength: 625 nm)
Research Area Plant Physiology Series
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.046 mmol/L
Detection Range 0.046-1 mmol/L
Precision inter-assay CV: 1.6-4.9% | intra-assay CV: 1.4-3.9%
Sample Volume 20 μL(tissue homogenate)
Assay Time 50 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
10% Epipremnum aureu root tissue homogenization 1
10% Pumpkin tissue homogenization 1
10% Corn leaf tissue homogenization 1
10% Green peppers tissue homogenization 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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Biotin

Chromogenic Biotin

Cyanine 3

Cyanine 5

Cyanine 5.5

Cyanine 7

Elab Fluor® Violet 450

Elab Fluor®430

Elab Fluor®488

Elab Fluor®568

Elab Fluor®594

Elab Fluor®647

Elab Fluor®680

Elab Fluor®700

Elab Fluor®750

Elab Fluor®Red 780

Elab Fluor®Violet 450

FITC

HRP

NHS-Biotin

NHS-LC-LC-Biotin

Sulfo-NHS-Biotin

Sulfo-NHS-LC-LC-Biotin

Unconjugated

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detection instrument

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