Plant Root Activity Assay Kit (E-BC-K855-S)
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For research use only.
| Detection Principle | The detection principle of the kit is to use 2,3,5-triphenyltetrazolium chloride (TTC) as the substrate, incubate for 1 to 3 hours, the dehydrogenase in the root can reduce TTC and generate 1,3,5-tiphenylformazan (TTF) which is insoluble in water, then TTF will be extracted from the root with organic solvent (ethyl acetate or acetone, etc.) By detecting the absorbance at 485nm, the amount of TTC reduction can be calculated, which represents the dehydrogenase activity and is used as an index of plant root activity. The kit is used for quantitative determination of plant root activity or dehydrogenase activity. |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (485 nm) |
| Research Area | Plant Physiology Series |
| Other Reagents Required | Double distilled water, Ethyl Acetate |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0 U/g wet weight |
| Detection Range | 0-0.67 U/g wet weight |
| Precision | inter-assay CV: 3% | intra-assay CV: 1% |
| Assay Time | 3 h 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Cymbidium | 1 |
| Black nightshade | 1 |
| Stone step grass | 1 |
| Bitter fleabane | 1 |
| Hemlock chervil | 1 |
| Oilseed rape | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
| Cat.No. | Product Name | Clone No. |
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