Plant Soluble Sugar Colorimetric Assay Kit (E-BC-K866-M)
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For research use only.
| Detection Principle | Carbohydrate is one of the important components of plant corpus, and is also the main raw material of metabolism and storage material. The kit is used for the determination of soluble monosaccharides, oligosaccharides and polysaccharides. It has the advantages of high sensitivity, quick convenience and suitable for the determination of microscale samples. The detection principle is anthrone colorimetry. Carbohydrate react with anthrone to produce colored material, which has the maximum absorption peak at 620 nm. The soluble sugar content can be determined by measuring the absorbance value. |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (610-630 nm, optimum wavelength: 620 nm) |
| Research Area | Plant Physiology Series |
| Other Reagents Required | Concentrated sulfuric acid, Ethyl acetate |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Detection Range | 0.003-1.5 mg/mL |
| Precision | inter-assay CV: 5.3% | intra-assay CV: 1.7% |
| Sample Volume | 200 μL(tissue homogenate) |
| Assay Time | 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mango tissue homogenate | 150-250 |
| 10% Fresh jujube tissue homogenate | 150-250 |
| 10% Grape tissue homogenate | 150-200 |
| 10% Corn tissue homogenate | 100-200 |
| 10% Apple tissue homogenate | 100-150 |
| 10% Banana tissue homogenate | 150-200 |
| 10% Cucumber tissue homogenate | 20-50 |
| 10% Tomato tissue homogenate | 30-60 |
| 10% Mushroom tissue homogenate | 1 |
| 10% Carrot tissue homogenate | 40-60 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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