Polyphenol Oxidase (PPO) Activity Assay Kit (E-BC-K259-M)
For research use only.
| Detection Principle | Polyphenol oxidase (PPO) can catalyze phenolic compounds into quinone substances. The latter has specific absorption at 410 nm. The activity of PPO can be calculated indirectly bymeasuring the OD value at 410 nm. |
| Synonyms | PPO |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (405-415 nm, optimum wavelength: 410 nm) |
| Research Area | Plant Physiology Series |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Precision | inter-assay CV: 8.7% | intra-assay CV: 4.6% |
| Sample Volume | 0.3 mL(tissue homogenate) |
| Assay Time | 25 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Ginger tissue homogenization | 1 |
| 10% Chinese yam tissue homogenization | 1 |
| 10% Corn tissue homogenization | 1 |
| 10% Pear tissue homogenization | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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