For research use only.
| Verified Samples |
Verified Samples in WB: 293T, Raji, Hela Verified Samples in IHC: Human cervical cancer |
| Dilution | WB 1:500-1:2000, IHC 1:25-1:100 |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide of human PRDX4 |
| Abbre | PRDX4 |
| Synonyms | AOE37 2, AOE37-2, AOE372, Antioxidant enzyme 372, Antioxidant enzyme AOE372, EC 1.11.1.15, PRDX 4, PRDX4, PRX 4, PRX4, Peroxiredoxin IV, Peroxiredoxin-4, Peroxiredoxin4, Prdx4, Prx IV, Prx-IV, PrxIV, Thioredoxin Peroxidase (A, Thioredoxin dependent peroxide reductase A0372 |
| Swissprot | |
| Calculated MW | 30 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm. Secreted. |
| Concentration | 0.8 mg/mL |
| Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer, Cell Biology |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | PRDX4(Peroxiredoxin-4) is also named as AOE37-2,Prx-IV and belongs to the AhpC/TSA family. PRDX4 is associated with acrosome formation during rat spermatogenesis and has a protective role in the male reproductive tract,because the phenotype of mice lacking this isoform includes testicular atrophy and increased sperm DNA damage. It is initially synthesized as a membrane-binding 31-kDa protein and processed into a 27-kDa secretory form and is discarded with the residual bodies. PRDX4 is a pentamer of dimers. |
Other Clones
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