Protein G Magnetic beads (EA-IP-016M)
For research use only.
Parameter Information | 1.Application Scope Applied in experiments such as immunoprecipitation of target proteins or their protein complexes for antibody binding. 2.Binding Properties High purity recombinant Protein G. 3.Binding substrate Magnetic beads, average particle size 3 μ M. 4.Loading Capacity 1 mL of magnetic bead suspension, containing approximately 10 mg of magnetic beads, covalently conjugated ≥ 0.6 mg of recombinant Protein G, binds ≥ 0.7 mg of human IgG. 5.Liquid storage composition Contain 0.2‰ sodium azide in PBS. |
Applications | IP/COIP |
Storage | 4℃, 12 months |
Shipping | ice bag |
Notes |
1. This product is limited to scientific research by professionals and cannot be used for clinical diagnosis or treatment. 2. For your safety and health, please wear laboratory clothes and disposable gloves for operation. 3. This product provides affinity magnetic beads in the form of a suspension. Before use, gently re-suspend the magnetic bead suspension and then use it as required. 4. This product needs to maintain a pH of 6-8, avoid high-speed centrifugation, drying, or freezing; Do not place the magnetic beads in a magnetic field for a long time, otherwise it may cause the beads to clump. 5. Before use, this product should be properly and thoroughly re-suspended, that is, inverted several times to mix the magnetic beads evenly. The mixing operation should be gentle and not violent vortex vibration, etc., to avoid antibody denaturation. 6. Do not centrifuge, freeze, or dry magnetic beads, do not sonicate the magnetic beads, and do not allow acid treatment of magnetic beads for more than 10 minutes. 7. When mixing the magnetic beads, please use methods such as gentle blowing with a pipette, gentle vortex, upside down, and shaking the bed to mix. 8. Relevant reagents for supporting use must be prepared by the laboratory itself. 9. Antibodies (IgG, IgM, IgA, IgD) of different species have different binding affinity with Protein G. Please read this manual carefully when using them Annex. |
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