PSMA Polyclonal Antibody (D-AB-10276L)
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For research use only.
| Verified Samples |
Verified Samples in WB: LNCaP Verified Samples in IHC: Human prostate cancer, Human kidney cancer, Mouse brain, Mouse kidney, Rat brain, Rat kidney |
| Dilution | WB 1:500-1:1000, IHC 1:100-1:300 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Recombinant Mouse PSMA Protein expressed by E.coli |
| Abbre | PSMA |
| Synonyms | Folylpoly-gamma-glutamate, Glutamate carboxypeptidase II (GCPII), Membrane glutamate carboxypeptidase (mGCP), N-acetylated-alpha-linked acidic dipeptidase I (NAALADase I), Prostate-s, Folate hydrolase 1, Glutamate carboxypeptidase 2, carboxypeptidase (FGCP) |
| Swissprot | |
| Calculated MW | 86 kDa |
| Observed MW |
110 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Signal Transduction, Cancer, Metabolism |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Prostate-specific membrane antigen (PSMA) is expressed in normal and malignant prostatic epithelium and in a subset of non-prostatic tissues. In prostate cancer,PSMA expression has been shown to correlate with disease progression,with highest levels expressed in hormone-refractory and metastatic disease. The cellular localization of PSMA is cytoplasmic and/or membranous. |
Other Clones
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Unconjugated
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