Purified Anti-Human CD28 Antibody[CD28.2], Functional Grade (E-AB-F11950)
![Purified Anti-Human CD28 Antibody[CD28.2], Functional Grade - 1](http://file.elabscience.com/assets/images/loading.png)
For research use only.
Conjugation: None (AF/LE)
APC Elab Fluor®488 Elab Fluor®647 FITC None (AF/LE) PE PE/Cyanine 5 PE/Cyanine 7 PE/Elab Fluor®594 PerCP/Cyanine 5.5 Unconjugated Show More
| Verified Samples | Verified Samples in FCM: Human peripheral blood red |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells), Activ Activ - Reported in the literature, Stim Stim - Reported in the literature |
| Isotype | Mouse IgG1, κ |
| Host | Mouse |
| Reactivity | Human |
| Applications | FCM, Activ, Stim |
| Clonality | Monoclonal |
| Abbre | CD28 |
| Synonyms | GPSAT, GYPA, HGpMiX, MNS, PAS2 |
| Swissprot | |
| Concentration | ≥ 1 mg/mL |
| Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Research Areas | Costimulatory Molecules, Immunology |
| Clone | CD28.2 |
| Conjugation | None (AF/LE) |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Shipping | Ice bag |
| background | CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
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