Purified Anti-Human CD3 Antibody[UCHT1], Functional Grade (E-AB-F12300)
For research use only.
Conjugation: None (AF/LE)
APC Biotin Elab Bright™Violet 421 Elab Bright™Violet 510 Elab Bright™Violet 650 Elab Fluor®488 Elab Fluor®700 Elab Fluor®Red 780 Elab Fluor®Violet 450 FITC None (AF/LE) PE PE/Cyanine 7 PerCP PerCP/Cyanine 5.5 Unconjugated Show More
| Verified Samples | Verified Samples in FCM: Human peripheral blood lymphocytes |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells), Depletion Depletion - Reported in the literature |
| Isotype | Mouse IgG1, κ |
| Host | Mouse |
| Reactivity | Human |
| Applications | FCM, Depletion |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human CD3 protein |
| Abbre | CD3 |
| Synonyms | CD3E, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E |
| Swissprot | |
| Concentration | ≥ 1 mg/mL |
| Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Purification Method | >98%, Protein A/G purified |
| Research Areas | Immunology |
| Clone | UCHT1 |
| Conjugation | None (AF/LE) |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Shipping | Ice bag |
| background | CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
| Cat.No. | Product Name | Clone No. |
|---|---|---|
| E-AB-F10010 | Purified Anti-Human CD3 Antibody[OKT-3], Functional Grade | OKT-3 |
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