Purified Anti-Human CD4 Antibody[OKT-4], Functional Grade (E-AB-F12310)
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For research use only.
| Alternate Names | Scd, T-cell surface antigen T4/Leu, T-cell surface glycoprotein CD, CD4, CD4mut, p55, Scd4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, T4, Leu-3, L3T4, W3/25, CD 4, CD4 (L3T4), CD4 antigen, CD4 antigen (p55), CD4 molecule, CD4 receptor, CD4+ Lymphocyte deficiency, included, L3T4, Leu3, Ly-4, Lymphocyte antigen CD4, MGC165891, OTTHUMP00000238897, T cell antigen T4, T cell antigen T4/LEU3, T cell differentiation antigen L3T4, T cell OKT4 deficiency, T cell surface antigen T4/Leu 3, T cell surface antigen T4/Leu3, T cell surface glycoprotein CD4, W3/25, W3/25 antigen |
| Clone | |
| Leadtime | Inquire |
| Verified Samples | Verified Samples in FCM: Human peripheral blood lymphocytes cell |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells);Activ Reported in the literaturee;Depletion Reported in the literature;Stim Reported in the literature |
| Background | CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) residue precursor protein with a 25 aa residue signal peptide, a 371 aa residue extracellular region containing four immunoglobulin homology domains, a 24 aa residue transmembrane domain and a 38 aa residue cytoplasmic domain. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
| Immunogen | Recombinant Human CD4 protein |
| Abbre | CD4 |
| Swissprot | |
| Host | Mouse |
| Reactivity | Human |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Isotype | Mouse IgG2b, κ |
| Isotype Control | Purified Mouse IgG2b, κ Isotype Control[MPC-11], Functional Grade |
| Applications |
FCM;Activ;Depletion;Stim
|
| Research Areas | Immunology;Stem Cells |
| Concentration |
≥ 1 mg/mL
|
| Conjugation | None (AF/LE) |
| Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
| Application References | Heike Danzer, et al. Elife. 2020 Jul 2:9:e55319. Christian Klammt, et al. Nat Immunol. 2015 Sep;16(9):961-9. |
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