Purified Anti-Human CD71 Antibody[CY1G4] (AN009670P)
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For research use only.
| Verified Samples | Verified Samples in FCM: Hela cells |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells) |
| Isotype | Mouse IgG2a, κ |
| Host | Mouse |
| Reactivity | Human |
| Applications | FCM |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human CD71 protein |
| Abbre | CD71 |
| Synonyms | IMD, sTfR, transferrin receptor protein, TFRC, CD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90, transferrin receptor, sTfR1, transferrin receptor protein 1, T9 |
| Swissprot | |
| Cellular Localization | Cell membrane, Membrane, Secreted |
| Tissue Specificity | Regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. Up-regulated upon mitogenic stimulation. |
| Concentration | ≥ 1 mg/mL |
| Buffer | Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling. |
| Purification Method | >98%, Protein A/G purified |
| Research Areas | Cardiovascular Biology, Immunology, Cancer, Metabolism, Stem Cells, Ferroptosis, CDs, Hematopoietic Stem Cell Markers, Neural Stem Cell marker, Serum Proteins |
| Clone No. | CY1G4 |
| Conjugation | Unconjugated |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The Transferrin Receptor (TfR or TfR-1, designated CD71) is a type 2 transmembrane glycoprotein expressed on erythroid progenitors, muscle cells and proliferating cells as a 188 kDa disulfide-linked homodimer of 95 kDa monomers (1‑4). As the major mediator of cellular iron uptake, it binds and internalizes diferric transferrin, allowing iron release at the low pH of the endosome. The human TfR cDNA encodes 760 amino acids (aa) including a 67 aa N-terminal intracellular domain, a 21 aa transmembrane domain, and a 672 aa extracellular domain (ECD) with helical, peptidase (nonfunctional), and ligand binding domains, including an RGD potential integrin binding site. Human TfR ECD shares 75‑80% aa identity with mouse, rat, feline, canine, equine, porcine and bovine TfR. A 679 aa alternately spliced form begins at aa 82 and is presumably secreted, while in an 804 aa form, 44 aa are inserted at aa 518 within the peptidase region. Most soluble TfR (sTfR) arises from trypsin proteolysis at aa 100, producing the circulating form of TfR. sTfR concentration in plasma or serum is proportional to total TfR and can be increased by iron deficiency. Erythroid progenitors, which use iron for hemoglobin synthesis, normally account for the bulk of total body TfR production. Since rapidly growing cells require iron to replicate DNA, cancer cells can express up to 5-fold more TfR than quiescent cells in the surrounding tissue. Antibody targeting of TfR can inhibit tumor cell proliferation and induce apoptosis. The hereditary hemochromatosis protein HFE competes with diferric transferrin for binding to TfR, and targets TfR for degradation rather than recycling. TfR has been reported to have ferritin-independent functions in T cell development, immunological synapse formation and galectin-3-mediated cell death, and to be a cell entry receptor for New World hemorrhagic fever arenaviruses. |
| Cat.No. | Product Name | Clone No. |
|---|---|---|
| E-AB-F1492A | Purified Anti-Human CD71 Antibody[HI160] | HI160 |
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