Purified Anti-Human/Monkey CD154/CD40L Antibody[5C8], Functional Grade (E-AB-F11280)
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For research use only.
| Alternate Names | T-BAM, TNFSF5, TRAP, gp39, CD40L |
| Clone | |
| Leadtime | Inquire |
| Verified Samples | Verified Samples in FCM: HEK293T cells transfected with pcDNA3.1 plasmid encoding Human CD154 gene |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells);Block Reported in the literaturee |
| Background | CD154 is a 39 kD TNF superfamily member also known as CD40 ligand, gp39, T-BAM, TRAP, and Ly-62. CD154 is an accessory molecule expressed predominantly on activated CD4+ lymphocytes that bind CD40. CD154 plays an important role in T-B cell costimulation. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
| Immunogen | Recombinant Human CD154 protein |
| Abbre | CD154 |
| Swissprot | |
| Host | Mouse |
| Reactivity | Human;Monkey |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Isotype | Mouse IgG2a, κ |
| Isotype Control | Purified Mouse IgG2a, κ Isotype Control[C1.18.4], Functional Grade |
| Applications |
FCM;Block
|
| Research Areas | Costimulatory Molecules;Immunology |
| Cellular Localization |
Secreted, membrane
|
| Concentration |
≥ 1 mg/mL
|
| Conjugation | None (AF/LE) |
| Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
| Application References | Ognjenka Nadazdin, et al. J Immunol. 2011 Nov 1;187(9):4589-97. |
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