Purified Anti-Human/Mouse CD44 Antibody[IM7], Functional Grade (E-AB-F11000)
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For research use only.
| Verified Samples | Verified Samples in FCM: C57/BL6 Mouse splenocytes |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells), Neut Neut- Reported in the literature |
| Isotype | Rat IgG2b, κ |
| Host | Rat |
| Reactivity | Human, Mouse |
| Applications | FCM, Neut |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human;Mouse CD44 protein |
| Abbre | CD44 |
| Synonyms | MDU2, MDU3, MIC4, CD44, CD44 antigen, CDw44, Epican, LHR, PGP-1, PGP-I, Phagocytic glycoprotein 1, Phagocytic glycoprotein I |
| Swissprot | |
| Concentration | ≥ 1 mg/mL |
| Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Purification Method | >98%, Protein A/G purified |
| Research Areas | Cell Adhesion, Cell Biology, Immunology, Stem Cells |
| Clone | IM7 |
| Conjugation | None (AF/LE) |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Shipping | Ice bag |
| background | CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation. None (Azide-Free,Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells. |
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